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1994-10-25
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Document 3186
DOCN M94A3186
TI HIV/HTLV western blot cross reactivity.
DT 9412
AU Constantine NT; Jones KS; Zhang X; Abdel-Hamid M; Dept. Pathology, Univ.
of Maryland Sch. of Med., Baltimore 21201.
SO Int Conf AIDS. 1994 Aug 7-12;10(1):133 (abstract no. PA0150). Unique
Identifier : AIDSLINE ICA10/94369389
AB OBJECTIVES: To assess the cross reactivity between HIV and HTLV by
Western blots (WB). METHODS: 100 sera, 92 classified as HIV inconclusive
(ELISA reactive but not confirmed by HIV WB), and 8 WB+ were tested
using one of two HTLV WB (1.2, Diagnostic Biotechnology; or HTLV-I/II,
Cambridge Biotech). 30 sera had been negative by HIV WB, while 62 had
produced indeterminate results. RESULTS: None of the sera tested were
confirmed positive for HTLV by WB. However, 19/92 (20%) of sera from
individuals with indeterminate HIV status showed cross reactivity with
HTLV. Cross reactivity was observed in 25% (16/62) of the HIV ELISA+/WB
indeterminate sera and 10% (3/30) of the ELISA+/WB negative sera. None
of the sera from HIV WB+ individuals exhibited cross reactivity to HTLV.
Among patients with reactivity to both HIV and HTLV, 11/16 (69%) reacted
to gag proteins from both viruses, while 0/16 reacted to env proteins
from both viruses. DISCUSSION AND CONCLUSIONS: We have previously
observed that HIV WB+ sera do not usually recognize HTLV proteins by WB.
Therefore, it appears that cross reactivity observed in sera from
individuals who are indeterminate for both viruses by WB are not due to
the recognition of HTLV proteins by antibodies against HIV. Possible
explanations include: (1) non viral antigens with epitopes similar to
retroviral proteins, and (2) expression of endogenous retroviral
sequences.
DE Blotting, Western/*METHODS Comparative Study Cross Reactions
Enzyme-Linked Immunosorbent Assay/METHODS Gene Products, gag/IMMUNOLOGY
Human HIV/ISOLATION & PURIF HIV Antibodies/*BLOOD HIV
Infections/BLOOD/*DIAGNOSIS/IMMUNOLOGY HTLV Antibodies/*BLOOD HTLV
Infections/BLOOD/*DIAGNOSIS/IMMUNOLOGY HTLV-BLV Viruses/ISOLATION &
PURIF Predictive Value of Tests MEETING ABSTRACT
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).